Synaptic vesicles transiently dock to refill release sites
Synaptic vesicles transiently dock to refill release sites
Abstract Synaptic vesicles fuse with the plasma membrane to release neurotransmitter following an action potential, after which new vesicles must ‘dock’ to refill vacated release sites. To capture synaptic vesicle exocytosis at cultured mouse hippocampal synapses, we induced single action potentials by electrical field stimulation then subjected neurons to high-pressure freezing to examine their morphology by electron microscopy. During synchronous release, multiple vesicles can fuse at a single active zone; this multivesicular release is augmented by increasing extracellular calcium. Fusions during synchronous release are distributed throughout the active zone, whereas fusions during asynchronous release are biased toward the center of the active zone. Immediately after stimulation, the total number of docked vesicles across all synapses decreases by ～40%. Between 8 and 14 ms, new vesicles are recruited to the plasma membrane and fully replenish the docked pool in a calcium-dependent manner, but docking of these vesicles is transient and they either undock or fuse within 100 ms. These results demonstrate that recruitment of synaptic vesicles to release sites is rapid and reversible.
Davis M Wayne、Vu Thien、Hujber Edward J、Chin Morven、Jorgensen Erik M、Adula Kadidia P、Watanabe Shigeki、Raychaudhuri Sumana、Kusick Grant F、Lippmann Kristina
Department of Biology and Howard Hughes Medical Institute, University of UtahNeurobiology Course, The Marine Biological Laboratory||Department of Biology and Howard Hughes Medical Institute, University of UtahNeurobiology Course, The Marine Biological Laboratory||Department of Biology and Howard Hughes Medical Institute, University of UtahDepartment of Cell Biology, Johns Hopkins University, School of MedicineNeurobiology Course, The Marine Biological Laboratory||Department of Biology and Howard Hughes Medical Institute, University of UtahNeurobiology Course, The Marine Biological LaboratoryDepartment of Cell Biology, Johns Hopkins University, School of Medicine||Neurobiology Course, The Marine Biological Laboratory||Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, School of MedicineDepartment of Cell Biology, Johns Hopkins University, School of MedicineDepartment of Cell Biology, Johns Hopkins University, School of Medicine||Biochemistry, Cellular and Molecular Biology Graduate Program, Johns Hopkins University, School of MedicineNeurobiology Course, The Marine Biological Laboratory
细胞生物学生物物理学生理学
Synaptic vesicle exocytosisneurotransmitter releasemultivesicular releaseasynchronous releasesynaptic vesicle dockingtransient dockingflash-and-freezezap-and-freeze
Davis M Wayne,Vu Thien,Hujber Edward J,Chin Morven,Jorgensen Erik M,Adula Kadidia P,Watanabe Shigeki,Raychaudhuri Sumana,Kusick Grant F,Lippmann Kristina.Synaptic vesicles transiently dock to refill release sites[EB/OL].(2025-03-28)[2025-05-19].https://www.biorxiv.org/content/10.1101/509216.点此复制
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