不同雌激素对大鼠颏舌肌细胞雌激素受体表达的影响比较

OBJECTIVES：To investigate and compare the effects of different estrogens on expression of ERs in genioglossus muscle cell. METHODS：the genioglossus muscle tissues were obtained from newborn (0-3d) SD rats. The myoblasts were cultured in incubator containing 1% O2 (hypoxia condition) for 24h, and estrogens were added into the culture medium simultaneously. There were also another two control groups: the blank control group (cultured in normoxia incubator) and the hypoxia control group. Western-blotting was performed to detect the expression of ERα and ERβ at protein level in genioglossus myoblasts of rats. Real-time RT-PCR was performed to detect the expression of ERα and ERβ at mRNA level in genioglossus myoblasts of rats. Results: The results of western-blot were generally accordance with those of real-time RT-PCR. As to the expression of ERα, it was significantly higher in E2 group, compared with the other groups (P<0.01). The expression in GEN group, RES group and RD group were observed to be higher than that of CIH group (P<0.05) and lower compared with NC group (P<0.01). But no statistically significant differences were observed among the GEN group, RES group and RD group (P>0.05). The expression of ERβ of the E2 group was still the highest (P<0.01). There was a significant increase of ERβ expression in GEN, RES and RD groups in comparison with the NC group (P<0.05), and the ERβ expression was higher in the RD group than that in the GEN group and RES group (P<0.05).Conclusion: genisten, resveratrol, and resveratrol dimer exhibit higher binding affinity for ERβ than for ERα.