纳米羟基磷灰石对肺癌细胞端粒酶基因表达的影响
Effect of HAP Nanoparticles on the Expression of Telomerase Gene of Lung Cancer Cells
研究羟基磷灰石纳米粒子(nHAP)对人肺癌A549细胞端粒酶基因表达的影响。采用均相沉淀法制备出稳定单分散的nHAP，应用透射电镜及X-射线衍射分析对其进行表征。nHAP作用A549细胞6h后，采用TREP-ELISA法检测肺癌A549细胞的端粒酶基因表达。结果表面明nHAP作用组的肺癌A549细胞的端粒酶活性值为0.694±0.029，而对照组为0.786±0.031，两组之间有显著性差异(P<0.05)。nHAP可使肺癌A549细胞的端粒酶基因表达下调。
o investigate the effect of HAP nanopaiticles (nHAP) on telomerase activation of the lung cancer cells. nHAP were synthesized homogeneous precipitation method and surveyed by TEM and x-ray diffraction analysis. After treated the lung cancer cells with nHAP for 6 hours, the expression of telomerase gene of lung cancer A549 cells was detected by TREP-ELISA. The value of A450 of lung cancer cells which telomerase gene expression was positive 0.694±0.029，and that of control group was 0.786±0.031, compared with control group(P<0.05). nHAP could reduce expression of telomerase gene of lung cancer A549 cells.
刘东雷、韩颖超、刘全、王建军、郑庆丰
肿瘤学基础医学分子生物学
羟基磷灰石纳米粒子肺癌细胞端粒酶
hydroxyapatite nanoparticleslung cancer cellstelomerase
刘东雷,韩颖超,刘全,王建军,郑庆丰.纳米羟基磷灰石对肺癌细胞端粒酶基因表达的影响[EB/OL].(2010-02-08)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201002-350.点此复制
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