PPARγ对鸡Perilipin1基因的转录调控研究

Studies in mammals have shown that, Perilipin1 was involved in adipocyte differentiation and regulation of glucose and lipid metabolism, and was closely related to the occurrence and development of obesity. Similar to this, chicken Perilipin1 plays important roles in regulating the process of adipocyte lipolysis, however,its transcriptional regulation mechanism in poultry has not been illuminated.Studies in mice have shown that Perilipin1 is a target gene of peroxisome proliferator activated receptor gamma (PPAR). The transcription of Perilipin1 gene is mainly regulated by PPARγ. In the present study, the report gene assay and electrophoretic mobility shift assaywere used to reveal the chicken Perilipin1 gene transcriptional regulation mechanism by PPARγ.Report gene results indicate that PPARγ could not activate chicken Perilipin1 gene promoter region from -1992 to -11. And the result of EMSA showed that PPARγ/RXRα could not bind to the -1912/-1882 region of chicken Perilipin1 gene promoter.Conclusion: there is no PPARγ binding site in the -1992/-11 region of chicken Perilipin1 gene.