肝细胞癌多基因甲基化研究

Objective To explain the aberrant methylation of tumor suppressor genes is common hepatocellular carcinoma. Methods Extracting cryopreservation of hepatocellular carcinoma, paracancerous tissues and normal liver tissues of DNA using sodium bisulfite has the characteristics of unmethylated cytosine to uracil without changing the methylated cytosine methylation primers were designed and the non methylated primers, and then respectively by PCR reaction. We examined the metastasis suppressor gene p15, SYK, TIMP-3, E-cadherin, RASSAFI and tumor related genes p53, RB1, WT1, P14, p16 promoter methylation status of the promoter region in 60 liver cancer, para cancer and 6 normal liver tissues. Results The frequency of promoter methylation of ten genes in 60 HCC varied from 8% in P53 to 90% in p14. The average methylated-gene numbers were significantly different between HCC and paired non-tumor tissues (P<0.05). Conclusion There are multiple gene methylation in HCC exist, and the level of methylation of liver cancer tissue than in noncancerous tissue high.