化学法改性溶菌酶抑菌性及结构研究

this study adopts the method of chemical modification to modified lysozyme, with EDAC as condensing agent, cinnamic acid ,caffeic acid and p-coumaric acid as modifier, form a certain degree of modified enzyme, and the properties of modified enzymes were studied. Results showed that the lytic activity of modified enzyme declined slightly, but the modified enzyme expanded the bactericidal spectrum, to the Escherichia coli and Pseudomonas aeruginosa, the minimal inhibition concentration of caffeic acid and p-coumaric acid modified enzyme was 0.5mg/mL, cinnamic acid modified enzyme was 0.75mg/mL, but the efficacy of Staphylococcus aureus and Micrococcus lysodeikticus was reduced slightly, the minimal inhibition concentration of caffeic acid and p-coumaric acid modified enzyme was 1.25 mg/mL, cinnamic acid modified enzyme was 1.25 mg/mL and 1.50 mg/mL, The secondary structure contents of three kinds modified enzymes were certain changes.