comparison of the chemical composition in Salvia miltiorrhiza Bunge from 5 different regions in Shaanxi province by direct injection ESI-Q-TOF-MS
ESI-Q-TOF-MS 因其扫描速度快、灵敏度高等优点，在中药化学成分的鉴定中应用广泛，且能提供碎片离子的精确质量数及可能的元素组成，不易受到药材的生长环境、提取条件等因素的影响，能够较为准确评价陕西不同地区的丹参药材质量。该文采用 ESI-Q-TOF-MS 直接进样分析方法对陕西商州、洛南、大荔、丹凤和铜川等五个地区丹参的化学成分进行比较分析，通过丹参水溶性和脂溶性成分的质谱丰度差异评价不同地区丹参化学成分含量的变化，对不同产地丹参的化学成分进行鉴定，综合分析选出最优丹参种植产地。结果表明：五地丹参均含有丹参酮ⅡA、隐丹参酮、丹参酮Ⅰ、二氢丹参酮、丹参酚酮、次丹参醌、丹参醌Ⅱ等 9 种脂溶性化学成分和丹参素、咖啡酸、阿魏酸、迷迭香酸、原紫草酸、紫草酸、原儿茶醛、丹酚酸 A、丹酚酸 B 等 9 种水溶性化学成分，其中丹参素钠、紫草酸、丹酚酸 B 和隐丹参酮的含量普遍较高，质谱丰度均大于 30%。但是随着种植环境不同，不同地区丹参的化学成分含量差异很大，如商州产丹参中含有较多具有生物活性的丹参酮类物质，其中丹参酮Ⅰ含量远远高于其他四个产地，质谱丰度达到 72.6 %，而其他地区丹参酮Ⅰ的质谱丰度仅为 1.8-11.3 %。研究表明，丹参质量按照地区排序为商州>铜川>大荔>洛南>丹凤。该方法为中药药材质量评价提供了科学、可靠、便捷的途径，为药材规格等级的制定提供了新的途径，为陕西丹参种植区域的选择提供重要信息。
ue to the advantages of scanning speed and high sensitivity, ESI-Q-TOF-MS is widely applicated to identify the chemical composition of traditional Chinese medicine. In addition, ESI-Q-TOF-MS can provide the precise mass number and possible element composition of fragmentation ions.Therefore, it can accurately evaluate the quality of salvia miltiorrhiza (Danshen) in different areas of Shaanxi province and provide the basis for quality control of Danshen. An direct injection ESI-Q-TOF-MS analysis method was developed to value the chemical composition in Danshen coming from Shangzhou, Luonan, Dali, Danfeng and Tongchuan in Shaanxi province. Through analyzing the MS abundance of water soluble and lipid soluble extracts, there is a obvious variation in chemical composition. The comparison among different abundance is benefit to select the optimum planting area of Danshen. Results show that all Danshen samples from 5 different locations have 9 kinds of lipid soluble chemical composition (Tanshinone I, Tanshinone A, Tanshinone B, Cryptotanshinone, Dihydrotanshinone, Danshenxinkun A, Danshenxinkun D, 2-Isopropyl-8 -methylphenanthrene-3,4-dione and 7-beta-hydroxy-8-13- abietadiene-11,12-dione) and 9 kinds of water soluble chemical composition (Danshensu, Caffeic acid, Ferulic acid, Rosmarinic acid, Prolithospermic acid, Lithospermic acid, Protocatechuic acid, Salvianolic acid A and Salvianolic acid B). The content of Danshensu, Lithospermic acid, Salvianolic acid B and Cryptotanshinone is higher than other chemical composition with a MS abundance of 30% at least. However, with the change of planting environment, the content of the same chemical constituents in Danshen varies greatly in different regions. Tanshinones with important biological activity more exist in Danshen coming from Shangzhou, especially the MS abundance of Tanshinone I is as high as 72.6%, which is much higher than that in other 4 regions ranging from 1.8% to 11.3%. According to the comprehensive comparison, the quality of Danshen is ranged by Shangzhou > Tongchuan > Dali> Luonan> Danfeng. The direct injection ESI-Q-TOF-MS method is not easily affected by the growth environment and extraction conditions of the medicinal materials. It provides a scientific, reliable and convenient way to evaluate the medicine quality and a new way to formulate the specifications and grades of traditional Chinese medicine. The system is not only suitable for the chemical composition of Danshen in Shaanxi province, but also easily transplanted and popularized in other Danshen planting places.
GE Ying、PAN Gao、WANG Xi、YAN Yong、ZHANG Yilin
药学生物科学现状、生物科学发展生物科学研究方法、生物科学研究技术
anshenESI-Q-TOF-MSchemical compositionabundancemedicine quality
GE Ying,PAN Gao,WANG Xi,YAN Yong,ZHANG Yilin.comparison of the chemical composition in Salvia miltiorrhiza Bunge from 5 different regions in Shaanxi province by direct injection ESI-Q-TOF-MS[EB/OL].(2018-10-26)[2025-08-02].https://chinaxiv.org/abs/201810.00258.点此复制
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