白藜芦醇对UVA辐射后成纤维细胞的影响

Objective: To investigate the effects of resveratrol on ultraviolet A (UVA)-induced cellular proliferation and inflammatory cytokines of human fibroblast. Method: The fourth generation human fibroblasts in logarithmic growth phase were used. Fibroblasts, which were respectively co-cultured with resveratrols in the concentrations of 0.01mmol/L, 0.1mmol/L, 0.5mmol/L, 1.0mmol/L for 6h, 24h, 48h, 72h, were tested using MTT to screen the optimal concentration. Immediately after irradiation with 10J/cm2UVA, the fibroblasts were treated with resveratrols and cultured for 6h, 24h, 48h, 72h. Cellular proliferation was tested using MTT, and the levels of IL-1α, IL-1β, IL-6 were measured using ELISA. Results: 0.01mmol/L and 0.1mmol/L resveratrols showed no influence on the cellular proliferation of normal human fibroblasts. 0.01mmol/L resveratrol prsent obviously protective effects on UVA-induced inhibition of cellular proliferation and secretion of IL-1α, IL-1β, IL-6. Conclusion: Resveratrol in 0.01mmol/L has photo-protective effects on UVA-induced fibroblasts, and the procedure is closely related to cellular proliferation and cytokines IL-1α, IL-1β, IL-6 secretion.