烟草根围丛枝菌根真菌的分离鉴定

his study investigated the diversity of arbuscular mycorrhizal (AM) fungi in the tobacco rhizosphere soil sampled from different environment of Guizhou in southwest China. Four genera and twenty AM fungi species were isolated by wet-seiving and centrifugation method, of these nine belong to Glomus, seven to Acaulospora, three to Gigaspora genera and one to Scutellospora. Soil DNA was extracted and AM specific fragments were amplified before the denaturing gradient gel electrophoresis (DGGE) analysis. Sequence analysis was shown to be correlated with the wet sieving and centrifugation result and the majority of the sequences recovered were Glomus species. Our research may give clues to the further exploration and utilization of the resources of AM fungi.