利用抑制性消减杂交结合表达谱芯片技术从无籽沙糖桔和沙糖桔中筛选自交不亲和相关基因

Self-incompatibility (SI) is an important trait of Citrus plants that is exploited by farmers for producing seedless fruit in Citrus. However, the molecular mechanism of SI in Citrus is not well understood. 'Wuzishatangju' (Citrus reticulata Blanco) (Self-incompatibility, SI) is an excellent seedless mandarin cultivar derived from a bud sport variation of a seedy 'Shatangju' cultivar (Self-compatibility, SC). Therefore, the two cultivars are excellent materials to study the mechanisms of SI and/or SC in Citrus. An integrative strategy combining eight suppression subtractive hybridization (SSH) libraries with cDNA microarray was used to study the different molecular mechanisms between SI 'Wuzishatangju' and SC 'Shatangju' (control) mandarins. A custom microarray screen resulted in a total of 1820 up- or down-regulated (Ratio≥3.0) obtained from 9810 positive clones. The expression of genes involved in embryonic development, ubiquitination pathway, Ca2+-signaling pathway, gibberellins (GAs) and auxin was significantly up-regulated in SI 'Wuzishatangju' compared with SC 'Shatangju' mandarin. The microarray analysis suggests that ubiquitin mediated proteolysis pathway might be involved in the SI reaction of 'Wuzishatangju.' Our research highlighted some candidate pathways that participate in the mandarin SI/SC reaction and could be beneficial for understanding the SI/SC systems evolution and breeding seedless citrus fruits in the future.