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纤维素酶酶活测定方法的研究与评价

Research and analysis of Cellulase activity measurement methods

中文摘要英文摘要

本实验以构建统一的工业化纤维素酶测活标准为目标,分析了国内外常用的测活标准IUPAC标准(Ghose, 1987)、中国农业行业标准(NY/T 912-2004)及各大公司的企业标准,结果显示采用IUPAC标准推荐的方法测定纤维素内切酶活力和总酶活力操作简单,稳定性、重复性较好,并进一步对国内生产厂家的一系列产品进行了测定分析,为工业化纤维素酶测活体系的统一奠定了基础。

In order to form a integrated standard for industrialized Cellulase,this experiment analyzed several commonly used enzyme activity test standard such as IUPAC standard, Chinese agriculture industry standard and many enterprises' norms. The results indicated that the enzyme activity measurements of endoglucanases and total cellulase suggested by IUPAC standard were the optimal for the reason of simple operations and favorable stability and repeatability. Furthermore, some domestic enterprises' products were also calibrated and analyzed, this action was conducive for the establishment of integrated industrial cellulase activity test system.

蒋彦、陈祥军

生物科学研究方法、生物科学研究技术生物化学纤维素化学工业

酶学纤维素酶测活方法酶活力统一标准

EnzymologyCellulaseMeasurement methodsCellulase activityIntegrated standard

蒋彦,陈祥军.纤维素酶酶活测定方法的研究与评价[EB/OL].(2011-01-26)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201101-1260.点此复制

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