人类乳腺癌组织中骨膜素的表达及其意义

bstract: Objective To investigate the expression of periostin(PN) in breast cancer tissues. Methods PN mRNA expression in breast tissues was measured by RT-PCR;. PN protein in breast tissues was evaluated by Western-blot. Immunohistochemistry (IHC) method (Envision method) was carried out to detect the expression of PN in breast cancer tissues of different clinical stages and metastasized lymph node,as well as benign breast disease.Results The expression of PN in cancer tissue was increased at both mRNA level (P<0.05) and protein level (P<0.05) compared to the benign and normal breast tissues. The PN protein expression level increased with cancer clinical stages as shown by IHC. Conclusion PN was overexpressed in breast cancer tissues than normal implies its role in the pathogenesis development of breast cancer.