蝎毒多肽干预慢性粒细胞白血病小鼠模型P210、JAK1表达

Objective: To investigate the peptide extracts from scorpion venom (PESVs) producing an effect on P210, JAK1 expression in CML-NOD/SCID mice. Methods：Firstly, K562 cells were injected into NOD/SCID mice, irradiated 270 cGy on body by137Cs beforehand，in order to establish the animal model of CML-NOD/SCID mice. Secondly, randomly to divide the mice of model establishment into four groups. GroupⅠ-Ⅲ were respectively cured by PESVs with different concentrations and Group Ⅳ was the model group injected by the physiological saline water. GroupⅤ was taken as control. Finally, to kill these mice after four weeks and to use Western Blot method to examine P210 expression in mice, simultaneously to use ELISA method to examine JAK1 expression. Results: The PESV has good inhibitory role in chronic myelocytic leukemia cells transmutation，which effectively suppress P210 expression and inhibit JAK1 expression. The effect of inhibiting them was related to PESV concentration. Conclusion: The PESV has good role in inhibiting chronic myelocytic leukemia cell transmutation by influencing P210, JAK1 and signal transduction mechanism.