Ubiquitin基因介导下的PRRSV GP5基因免疫特性研究

he ubiquitin-proteasome pathway is an efficient pathway for protein degradation. It’s mainly responsible for the selective degradation of many proteins in eukaryotic cells. In order to evaluate the immunity of GP5 protein gene of porcine respiratory and reproductive syndrome virus (PRRSV), the GP5 protein gene was amplified with PCR and cloned into both pCMV-Ub and pCMV plasmid, respectively, and two recombinant plasmids, pCMV-Ub-GP5 and pCMV-GP5, were constructed and identified. Mice were immunized with the two kinds of plasmids. The serum antibodies and cellular immune response of the mice were detected. The result s showed that there were not much more differences between the two plasmids in antibody response, but the fusion gene DNA induced stronger cellular immune response than single gene DNA did, which indicated that Ub conjugation did improve the cellular immune response to GP5 in mice.