肥大细胞在乌鸡免疫器官中发育变化研究

【Objective】To investigate the developmental changing of the mast cell in avian immune organs.【Method】Using the wax section and toluidine blue staining method to study the role the mast cell plays in silkie immune organs based on the observation of histological structure of these organs.【Result】(1) The mast cells in silkie bursa of Fabricius are mainly distributed in epithelium and a small amount of them also exist in cortex. With no obvious nucleolus, they are purple in the shape of round or oval and differ slightly in size. There are less of mast cells in 14-day-old silkies while more in silkies that are 49 days old. (2) In silkie spleen, the mast cells are mainly in red pulps with little in white pulps. Some also exist along the blood vessels and trabeculae of red pulps and in splenic corpuscle. Same in size, these mast cells of the color of bluish violet are round or oval with a small amount of irregular shape. With a small diameter, their nucleoli are not obvious and there are protuberances in the ambitus of some cells. Silkies of 14 and 35 days old have more mast cells and those of 112 days old have the most. (3)Silkie thymus gland contains a great deal of mast cells, mainly distributed in medulla around thymus corpuscles, some in cortex and where cortex meets medulla and a small amount in the interstitial part of adjacent thymic lobules. The round or oval mast cells are of the color of bluish violet, same in size and with no obvious nucleolus. The number of mast cells differs in silkies of different ages. There are less in 63-day-old silkies but more in those of 112 days old.【Conclusion】As a kind of regular cell in connective tissue, the mast cells are usually distributed in the lymphoid tissue of bird immune organs and some can also be seen in groups along small blood and lymphatic vessels. The number of mast cells differ in different animals and organs.