Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord
Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord
Summary Spinal neurons are highly heterogeneous in location, transcriptional identity and function. To understand their contributions to sensorimotor circuits, it is essential to map the positions of identified subsets of neurons in relation to others throughout the spinal cord (SC), but we lack tools for whole SC sample preparation, imaging and in toto analysis. To overcome this problem, we have (1) designed scaffolds (SpineRacks) that facilitate efficient and ordered cryo-sectioning of the entire SC in a single block, (2) constructed a 3D reference atlas of adult mouse SC and (3) developed software (SpinalJ) to register images of sections and for standardized analysis of cells and projections in atlas space. We have verified mapping accuracies for known neurons and demonstrated the usefulness of this platform to reveal unknown neuronal distributions. Together, these tools provide high-throughput analyses of whole mouse SC and enable direct comparison of 3D spatial information between animals and studies.
Mason Carol、Dodd Jane、Ng David、Fiederling Felix、Hammond Luke A.
Department of Pathology and Cell Biology, Zuckerman Institute, Columbia UniversityDepartments of Physiology & Cellular Biophysics and Neuroscience, Zuckerman Institute, Columbia UniversityZuckerman Institute, Columbia UniversityDepartments of Physiology & Cellular Biophysics and Neuroscience, Zuckerman Institute, Columbia UniversityZuckerman Institute, Columbia University
生物科学研究方法、生物科学研究技术普通生物学细胞生物学
Spinal cordreference atlastissue embeddingSpinalJSpineRackneuronmappinghistology3Dposition
Mason Carol,Dodd Jane,Ng David,Fiederling Felix,Hammond Luke A..Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord[EB/OL].(2025-03-28)[2025-07-02].https://www.biorxiv.org/content/10.1101/2021.05.06.443008.点此复制
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