aveolin-1基因真核表达载体的构建与表达鉴定

Objective To construct the recombinant eukaryotic expression vector of Caveolin-1 gene and observe its expression in eukaryotic cells. Methods Caveolin-1 gene full-length cDNA (633bp) from the brain tissue of rats was amplified by RT-PCR ; After double- enzyme digestion and gel recovery, Caveolin-1 gene was cloned into pYr-adshuttle-3 eukaryotic expression vector to construct pYr-ads-cav1 recombinant vectors. Vector pYr-ads-cav1 was then transfected into HEK293 cells to identify the expression of Caveolin-1 gene. Results Recombinant vectors pYr-ads-cav1 was confirmed to be successfully constructed through enzyme digestion and sequencing analysis. And Caveolin-1 gene could express in HEK293 cells which was testified by RT-PCR. Conclusion Expression vector pYr-ads-cav1 was constructed successfully.