miRNA-196b过表达对K562细胞增殖、凋亡及Survivin、Cox-2表达的影响

o investigate the effects of miRNA-196b overexpression on proliferation, apoptosis and Cox-2，survivin genes expression of K562 cells. Methods Four groups including miRNA-196b, siRNA-ABL, Imatinib（IM） and K562 control group were set up in this study. The cell proliferation and apoptosis were measured by CCK-8 assay and Annexin V-FITC/PI, respectively. The expression of Cox-2 and survivin genes at the mRNA level was detected by RT-qPCR. Results The proliferation of K562 cells could be significantly inhibited by miRNA-196b overexpression; Compared of the four groups of apoptosis rate, miRNA-196b group significantly higher than K562 and siRNA-ABL groups, but less than imatinib group (P < 0.05). The expression of survivin gene in miRNA-196b was donwregulated(P < 0.05),but the expression of cox-2 gene in miRNA-196b group had no significant difference（P>0.05）.Conclusion The miRNA - 196b plays an important role in K562 cells proliferation inhibition and apoptosis；Overexpression of miRNA-196b can down-regulate surviving gene expression, provide some basis for miRNA-196b as a therapeutic target for chronic myelogenous leukemia.