转Bt基因抗虫棉抗虫性评价与新品系筛选

IAbstract: The insect-resistant was evaluated in fourteen transgenic Bt gene cotton varieties by marker gene expression, Bt gene expression, Bt Protein expression and the leaf feeding method in this study. The result shown that marker gene expression and Bt gene expression was inconsistent in some transgenic insect-resistant cotton. The difference was found between Bt protein expression and insect-resistant in fourteen varieties. The rule of Bt protein expression from high to low were, in order, leaf, boll, flower. And bud stage, blossing and boll-forming stages, boll-forming stages. Five good varieties including NDP7、NDP8、NDP9、NDP12、NDP14 were obtained.