香鱼akirin1基因的特征分析与表达

kirin, a conserved nuclear factor, is associating with immune functions in animal via regulating cytokine transcription and immune cell chemotaxis. Here, we cloned a akirin1 cDNA sequnece (Paakirin1) from ayu, Plecoglossus altiveli. The Paakirin1 is 1817 bp in length encoded a 182 aa polypeptide with a molecular weight of 20.68 kDa. Multiple sequence alignment analysis showed that all akirin1 genes in teleost possessed nuclear localization signal. Phylogenetic analysis showed that fish akirin1 genes grouped tightly into one big cluster, and Paakirin1 had the most closest relationship with those of the salmonidae family. Semi-quantitative PCR data showed that Paakirin1 was mainly expressed in head-kidney, spleen, intestine, and monocytes/macrophages. After Vibrio anguillarum infection, Paakirin1 mRNA was up-regulated in head-kidney and spleen, while down-regulated in intestine. Furthermore, Paakirin1 mRNA was also up-regulated in monocytes/macrophages after LPS or PolyI:C treatment. In summary, Paakirin1 expression is associated with immune stimulation, may suggesting its function in immune regulation.