电针对糖尿病小鼠骨骼肌纤维类型分化的影响

Objective: To observe the effect of electro-acupuncture on skeletal muscle fiber transformation in diabetic mice. Methods: 45 C57 mice were randomly divided into control group, drug group and EA group, with 15 mice in each group. The high fat diet combined with small dose of STZ was used to induce the model of type 2 diabetes. Mice in the EA group were treated by electro-acupuncture in point "Housanli" and "Neiting", drug group mice were lavaged with metformin (250 mg / kg body weight) , mice in control group were lavaged with normal saline (2ml / kg body weight), once daily for 4 weeks. Soleus muscle was taken to detect the expression of mRNA and protein of MHC, a marker of skeletal muscle type. Results: Compared with the drug group and the control group, the expression of mRNA and protein of MHCI in mice with electro-acupuncture was significantly increased (P < 0.01), while the expression of mRNA and protein of MHCIIb was significantly decreased (P < 0.01). Conclusion: Electro-acupuncture can induce the expression of MHCI in diabetic skeletal muscle , and promote the transformation of skeletal muscle to type I fiber.