蝎毒多肽提取物抑制DU-145 细胞COX-2 和 MMP-9表达的研究
Polypeptide extract from scorpion venom （PESV）downregulates the expression of COX-2 and MMP-9 inDU-145 cell lines
目的 研究蝎毒多肽提取物（peptide extract from scorpion venom，PESV）对雄激素非依赖性人前列腺癌细胞株DU-145COX-2和MMP-2表达的影响，进一步探讨其抗血管生成的分子机制，为抗前列腺癌骨转移提供有效的治疗手段。方法 采用免疫细胞化学方法检测PESV对前列腺癌细胞株DU-145COX-2、MMP-9的表达影响，应用RT-PCR检测PESV对MMP-9在mRNA水平表达的影响。结果蝎毒多肽提取物（40μg/ml）作用于前列腺癌细胞后，COX-2、MMP-9蛋白表达水平下调，MMP-9在mRNA水平亦下降。结论 蝎毒多肽提取物（PESV）通过抑制前列腺癌细胞血管生成因子COX-2的表达而发挥其抗血管生成作用，具有临床应用价值.
Objectives To identify the effect of Polypeptide extract from scorpion venom（PESV） on the expression of cyclooxygenase-2(COX-2) and matrix metalloproteinase-9(MMP-9) and get insights into the mechanism of PESV inhibiting metastasis of prostatic cancer.Methods Immunohistochemistry was used to study the change of COX-2 and MMP-9 in DU-145 cell lines treated with PESV(40μg/ml). MMP-9 mRNA was detected with methods of Rt-PCR.Results The expression of proreins COX-2 and MMP-9 were downregulated ;MMP-9 mRNA was depressed compared with the control group. Conclutions PESV is of potent anti-angiogenic activity through inhibiting the expression of COX-2. PESV could be regarded as a candidate for tumor angiogenesis inhibitor .
宋守琴、张月英、贾青、张维东、黄山英、王兆朋、王朝霞
肿瘤学基础医学分子生物学
蝎毒前列腺肿瘤OX-2MMP-9
scorpion venomProstatic neoplasmacyclooxygenase-2matrix metalloproteinase-9
宋守琴,张月英,贾青,张维东,黄山英,王兆朋,王朝霞.蝎毒多肽提取物抑制DU-145 细胞COX-2 和 MMP-9表达的研究[EB/OL].(2006-04-21)[2025-07-21].http://www.paper.edu.cn/releasepaper/content/200604-309.点此复制
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