高效液相色谱法定量检测玉米油中玉米赤霉烯酮的溶剂萃取预处理方法研究

In this study, a solvent extraction pretreatment method for determination of zearalenone (ZEN) in refined maize oil by HPLC (SE-HPLC) was established and assessed. The optimal conditions of solvent extraction pretreatment were obtained as follows: 90% acetonitrile water solution as extraction solution, ratio of material to liquid 1:4 (m/V，g/mL) and ultrasonic extracting time 20 min. The extraction pretreatment conditions ensured that the chromatographic peaks of ZEN quantitative determination were separated from the baseline and ZEN was accurately quantified. The results showed that detection limit of SE-HPLC method was 4.5 μg/kg. The mean recoveries of the target compounds (spiked at three concentration levels:10 μg/kg, 100 μg/kg and 1000 μg/kg) were 77.9%, 105.7% and 95.0%, with the relative standard deviations (RSDs) were ranged from 2.05% to 8.43%. Through comparative analysis, the method is better than traditional immune affinity column -HPLC method.