Changes in Ovary Transcriptome and Alternative splicing at estrus from Xiang pigs with Large and Small Litter Size
Changes in Ovary Transcriptome and Alternative splicing at estrus from Xiang pigs with Large and Small Litter Size
Abstract Background/AimsLitter size is one of the most important reproductive traits in pig breeding, which is affected by multiple genes and the environment. Ovaries are the most important reproductive organs and have a profound impact on the reproduction efficiency. Therefore, genetic differences in the ovaries may contribute to the observed differences in litter size. Although QTLs and candidate genes have been reported to affect the litter size in many pig breeds, however, the findings cannot elucidate the marked differences of the reproductive traits between breeds. The aim of present work is to elucidate the mechanisms of the differences for the reproductive traits and identify candidate genes associated with litter size in Xiang pig breed. MethodsThe changes in ovary transcriptome and alternative splicing were investigated at estrus between Xiang pigs with large and small litter size by RNA-seq technology. The RNA-seq results were confirmed by RT-qPCR method. ResultsWe detected 16,219 - 16,285 expressed genes and 12 types of alternative splicing (AS) events in Xiang pig samples. A total of 762 differentially expressed genes were identified by XL (Xiang pig group with larger litter size) vs XS (Xiang pig group with small litter size) sample comparisons. A total of 34 genes were upregulated and 728 genes were downregulated in XL ovary samples compared with the XS samples. Alternative splicing (AS) rates in XL samples were slightly lower than that observed in XS samples. Most of differentially expressed genes were differentially regulated on AS level. Eleven candidate genes were potentially identified to be related to Xiang pig fecundity and litter size, which may be closely related to the gonad development, oocyte maturation or embryo quality. ConclusionThe significant changes in the expression of the protein-coding genes and the level of alternative splicing in estrus ovarian transcriptome between XL and XS groups probably are the molecular mechanisms of phenotypic variation in litter size.
Ruan Yiqi、Wang Jiafu、Zhang Fuping、Tang Liangting、Niu Xi、Huang Shihui、Yi Fanli、Liu Chang、Ran Xueqin、Mao Ning、Li Sheng
College of Animal Science / Institute of Agro-Bioengineering and Key Laboratory of Plant Resource Conservative and Germplam Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Animal Science / Institute of Agro-Bioengineering and Key Laboratory of Plant Resource Conservative and Germplam Innovation in Mountainous Region (Ministry of Education), Guizhou University||Tongren UniversityCollege of Animal Science / Institute of Agro-Bioengineering and Key Laboratory of Plant Resource Conservative and Germplam Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Animal Science / Institute of Agro-Bioengineering and Key Laboratory of Plant Resource Conservative and Germplam Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Animal Science / Institute of Agro-Bioengineering and Key Laboratory of Plant Resource Conservative and Germplam Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Animal Science / Institute of Agro-Bioengineering and Key Laboratory of Plant Resource Conservative and Germplam Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Animal Science / Institute of Agro-Bioengineering and Key Laboratory of Plant Resource Conservative and Germplam Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Animal Science / Institute of Agro-Bioengineering and Key Laboratory of Plant Resource Conservative and Germplam Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Animal Science / Institute of Agro-Bioengineering and Key Laboratory of Plant Resource Conservative and Germplam Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Animal Science / Institute of Agro-Bioengineering and Key Laboratory of Plant Resource Conservative and Germplam Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Animal Science / Institute of Agro-Bioengineering and Key Laboratory of Plant Resource Conservative and Germplam Innovation in Mountainous Region (Ministry of Education), Guizhou University
畜牧、动物医学遗传学分子生物学
Litter sizeTranscriptomeAlternative splicingEstrusOvaryXiang pig
Ruan Yiqi,Wang Jiafu,Zhang Fuping,Tang Liangting,Niu Xi,Huang Shihui,Yi Fanli,Liu Chang,Ran Xueqin,Mao Ning,Li Sheng.Changes in Ovary Transcriptome and Alternative splicing at estrus from Xiang pigs with Large and Small Litter Size[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/547810.点此复制
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