Persistence of SARS CoV-2 S1 Protein in CD16+ Monocytes in Post-Acute Sequelae of COVID-19 (PASC) Up to 15 Months Post-Infection
Persistence of SARS CoV-2 S1 Protein in CD16+ Monocytes in Post-Acute Sequelae of COVID-19 (PASC) Up to 15 Months Post-Infection
ABSTRACT The recent COVID-19 pandemic is a treatment challenge in the acute infection stage but the recognition of chronic COVID-19 symptoms termed post-acute sequelae SARS-CoV-2 infection (PASC) may affect up to 30% of all infected individuals. The underlying mechanism and source of this distinct immunologic condition three months or more after initial infection remains elusive. Here, we investigated the presence of SARS-CoV-2 S1 protein in 46 individuals. We analyzed T-cell, B-cell, and monocytic subsets in both severe COVID-19 patients and in patients with post-acute sequelae of COVID-19 (PASC). The levels of both intermediate (CD14+, CD16+) and non-classical monocyte (CD14Lo, CD16+) were significantly elevated in PASC patients up to 15 months post-acute infection compared to healthy controls (P=0.002 and P=0.01, respectively). A statistically significant number of non-classical monocytes contained SARS-CoV-2 S1 protein in both severe (P=0.004) and PASC patients (P=0.02) out to 15 months post-infection. Non-classical monocytes were sorted from PASC patients using flow cytometric sorting and the SARS-CoV-2 S1 protein was confirmed by mass spectrometry. Cells from 4 out of 11 severe COVID-19 patients and 1 out of 26 also contained SARS-CoV-2 RNA. Non-classical monocytes are capable of causing inflammation throughout the body in response to fractalkine/CX3CL1 and RANTES/CCR5. SummarySARS CoV-2 S1 Protein in CD16+ Monocytes In PASC
Francisco Edgar B.、Rodrigues Hallison、Yogendra Ram、Hall Eric、Parikh Purvi、Guevara-Coto Jose、Pise Amruta、Mora Javier、Long Emily、Patterson Bruce K.、Sacha Jonah B、Herrara Monica、Mora-Rodr¨aguez Rodrigo A、Chang Xaiolan
IncellDx IncIncellDx IncLawrence General HospitalBio-Rad LaboratoriesNYU Langone HealthLab of Tumor Chemosensitivity, CIET / DC Lab, Faculty of Microbiology, Universidad de Costa Rica||Department of Computer Science and Informatics (ECCI), Universidad de Costa RicaIncellDx IncLab of Tumor Chemosensitivity, CIET / DC Lab, Faculty of Microbiology, Universidad de Costa RicaIncellDx IncIncellDx IncVaccine & Gene Therapy Institute and Oregon National Primate Research Center, Oregon Health & Science UniversityBio-Rad LaboratoriesLab of Tumor Chemosensitivity, CIET / DC Lab, Faculty of Microbiology, Universidad de Costa RicaVaccine & Gene Therapy Institute and Oregon National Primate Research Center, Oregon Health & Science University
医学研究方法基础医学
COVID-19PASCSARS CoV-2 S1 Proteinnon-classical monocytesCCR5fractalkine
Francisco Edgar B.,Rodrigues Hallison,Yogendra Ram,Hall Eric,Parikh Purvi,Guevara-Coto Jose,Pise Amruta,Mora Javier,Long Emily,Patterson Bruce K.,Sacha Jonah B,Herrara Monica,Mora-Rodr¨aguez Rodrigo A,Chang Xaiolan.Persistence of SARS CoV-2 S1 Protein in CD16+ Monocytes in Post-Acute Sequelae of COVID-19 (PASC) Up to 15 Months Post-Infection[EB/OL].(2025-03-28)[2025-05-09].https://www.biorxiv.org/content/10.1101/2021.06.25.449905.点此复制
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