口服葛根芩连汤后黄芩活性成分在大鼠体内的组织分布

Objective To investigate the tissue distribution of heart,liver,spleen,lung,kidney, stomach, and small intestine of active ingredient of Radix Scutellaria after oral administration of Gegeng Qinlian Decoction. Method LC-MS/MS was used;Column:Shiseido CAPCELL PAK C18 (100mm×2.0mm i.d., 5μm); mobile phase: 0.1% formic acid acetonitrile-0.1% formic acid water with a linear gradient; Electrospray ionization(ESI) source was applied under the postive ion mode and multi-reaction monitoring mode(MRM) was used to qualify the analytes. Results The average recoveries of four active ingredients were more than 75.5%,and the RSDs of intra- and inter-day precisions of all analytes were less than 11.9%.The concentration of each component is quite different in various tissues and organs. Conclusions The major distribution organs of Baicalin,Baicalein,Wogonoside,Wogonin in rats are liver,stomach and small intestine.