Differential vaginal Lactobacillus species metabolism of glucose, L and D-lactate by 13 C-nuclear magnetic resonance spectroscopy
Differential vaginal Lactobacillus species metabolism of glucose, L and D-lactate by 13 C-nuclear magnetic resonance spectroscopy
Abstract IntroductionCervicovaginal dysbiosis can lead to infection-associated spontaneous preterm birth. ObjectiveTo determine whether vaginal Lactobacillus species, L. crispatus and L. jensenii, differentially metabolise glucose, L- and/or D-lactate to propagate their survival/dominance. MethodsBacteria were incubated anaerobically for 24h at 37°C, with 13Cu-glucose, 13C3-D-lactate or 13C3-L-lactate (singularly or combined) for 24h. 13C-spectra were acquired using a 9.4T NMR spectrometer. ResultsL. crispatus and L. jensenii (n=6 each) metabolised 13C-glucose to 13C-lactate and 13C-acetate. L. jensenii converted more 13C3-D- or 13C3-L-lactate to 13C-acetate than L. crispatus, p<0.001. ConclusionConversion of glucose and lactate to acetate by L. jensenii compared to L. crispatus, suggests a possibly important pathomechanism of dysbiosis and infection-associated spontaneous preterm birth.
Amabebe Emmanuel、Reynolds Steven、Anumba Dilly O.
Department of Oncology and Metabolism, University of Sheffield, Level 4, Jessop WingDepartment of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Royal Hallamshire hospitalDepartment of Oncology and Metabolism, University of Sheffield, Level 4, Jessop Wing
基础医学妇产科学微生物学
Preterm birthvaginal lactobacillilactateacetate13C-NMR
Amabebe Emmanuel,Reynolds Steven,Anumba Dilly O..Differential vaginal Lactobacillus species metabolism of glucose, L and D-lactate by 13 C-nuclear magnetic resonance spectroscopy[EB/OL].(2025-03-28)[2025-05-14].https://www.biorxiv.org/content/10.1101/2020.03.10.985580.点此复制
评论