拟南芥转录调节因子AtBT4的生物信息学分析

We obtained a transcriptional regulatory factors in preliminary research which is a protein resist Botrytis cinerea. The purpose of this study was to analysis of the gene related information of the bioinformatics. With the bioinformatics analysis, the AtBT4 gene was 1152 bp, encoding 383 amino acids, the predicted AtBT4 protein' s molecular formula was C 1936 H 3097 N 561 O 543 S 28, whose relative molecular mass was 43.82 kDa and isoelectric point was 9.33. It was predicted that this protein was hydrophilic and non-secretary protein, and there was no signal peptide. And there were fourteen serine sites, one threonine sites, and three tyrosine sites.Characteristic prediction results indicated that the secondary structure of AtBT4 protein mainly included α-helix (55.35%), Beta turn (8.36%), Extended strand (10.70%) and random coil (25.59%). We predicted the tertiary structure of AtBT4 and obtain a three-dimensional structural model of the protein. We analyzed the promoter of AtBT4 gene and found the promoter contain A-box, CCGTCC-box, O2-site, GCN4-motif, GT1-motif, TCT-motif, I-Box, TGACG-motif. We found that the protein can interact with CIPK 21, COR27, TAG1 protein and interact with the gene of SYNC1, SYNC2, AHB1, AHB2, EULS3, CYSC1, CDC25, BZR2. The results is benefit for further research molecular mechanism of the protein which disease resistance in Arabidopsis.