黄瑾（Hibiscus tiliaceus）内生真菌Penicillium sp. LD-68的次级代谢产物及其生物活性研究

Objective To investigate the antitumor bioactive ingredient of a endophytic fungus Penicillium sp. LD-68 isolated from Guang Xi mangrove plant Hibiscus tiliaceus L.. Methods The extract of the fungus was purified by solvent extraction, column chromatography and preparative HPLC guided by the bioassay results. Their structures were established by physicochemical properties and spectroscopic analysis. The antitumor activities were evaluated by SRB method and MTT method. Results Five compounds were identified as curvularin (1), dehydrocurvularin (2), 11-β-Hydroxy-12-Oxocurvularin (3), 11-β-Hydroxycurvularin (4), 11-α-Hydroxycurvularin (5). Compounds 1-5 showed inhibitory activities to four kinds of human tumor cell lines including A549, hela, BEL-7402 and K562, and compounds 2-5 have more potential antitumor activities against hela cells, with IC50 values of 3.99, 7.75, 10.00 and 5.10μM, respectively. Conclusion Compounds 3-5 are report ed their cytoxic activities against the four kinds of human tumor cell lines, A549, hela, BEL-7402 and K562 for the first time, and exhibiting more selectivities to hela cell line.