体外诱导成年大鼠骨髓间质细胞向schwann细胞转化的实验研究

Objective Investigation of the mechanism and methods of adult rat bone marrow stromal cells’(MSCs) differentiation into schwann cells . Methods Adult rat MSCs were treated with the substance extracted from denatured nerve and nonserum culture medium with trophic factors like Leukemia inhibitory factor (LIF) respectively. Observing the morphologic change of MSCs and then identify them by immunocytochemistry methods. Results After differentiation, MSCs’ shape changed greatly, and turned from polygonal cells into bipolar cells like schwann cells. Immunocytochemistry show that S-100, GFAP stain postive in LIF differented cells.. Conclusion There are necessary constitutes that can differentiate MSCs into schwann cells in the secretion of denatured peripheral nerve; LIF is the important constitute of the secretion for MSCs to differentiate into schwanns.