补肾活血中药对高糖条件下纯化培养的视网膜神经节细胞Glu释放量的影响

o investigate the effect of serum with TCM for bushenhuoxue on the Glu release of purified retinal ganglion cells in high glucose level conditions Methods: Two monoclonal antibodies,anti-rat SIRP(OX-41)against rat macrophage and antibody against rat Thy-1.1(OX-7) were used to purify and characterize RGCs from rat neonates by means of two-step filtration. Purified RGCs were cultured in steadily high glucose conditions﹑glucose fluctuated conditions and normal control conditions ,bushenhuoxue medicine-containing serum was added to the culture medium. The Experiment was assigned into normal control group﹑TCM interventing group﹑steadily high glucose group﹑steadily high glucose group intervened with TCM﹑glucose fluctuated group﹑glucose fluctuated group intervened with TCM; Deteced the content of the glutamate(Glu) in the extracellular fluid with a full- automatic biochemical analyzer. And then the data obtained was treated with SPSS 13.0. Results：The release of Glu was obviously increased in glucose fluctuated conditions at 24 hours（P＜0.05）, and the release of Glu was obviously decreased by TCM intervention both in steadily high glucose conditions at each period and glucose fluctuated conditions at 24 hours and 72 hours（P＜0.05）. Conclusion: Bushenhuoxue therpy could decreas the Glu release from RGCs cultured in both steadily high glucose conditions and glucose fluctuated conditions, accordingly it might be one of the important mechanisms of bushenhuoxue therpy could prevent and cure DRP.