大豆Glyma08g02580及其同源蛋白的生物信息学分析

Genes containing the WRKY domain are plant-specific transcriptional regulators and are expressed in various developmental stages and tissuses. The most archaic WRKY transcription factors, which may originate some 1.5-2 billion years ago in eukaryotes,contain two highly conserved WRKY domain. Althogh all WRKY proteins regulate mainly the expression of target genes by specifically binding to the (T)(T)GACC(A/T) (W box) sequences of their promoter,the biological function of each member is specific. In the study,we performed a bioinformatics analysis of WRKY family genes in Glycine max. Based on bioinformatics methods, taking advantage of soybean database combined with public database (NCBI，TIGR，TAIR)，we identified 196 WRKY protein from soybean genome and download gene expression profile from GEO database. Meanwhile, we identified 197 homology protiens of Glyma08g02580.1 in other plants, such as Arabidopsis ,Oryza sativa.We analyzed the phylogenetic of 197 WRKY family protein sequence and domain characteristics.The results of this investigation could be definitely provide a significant foundation for further research on function analysis of Glycine max WRKY gene family.