柑橘木虱Diaphorina citri Kuwayama可培养性内生细菌的分离鉴定

In this paper, by using four factors and three levels L9 （34） method , the medium can be optimized to separation the endophytic bacteria of Citrus psyllid ,The results show that a variety of test operating conditions, consistent with the culture conditions, various factors influence the design level, the medium of TSB, pH 7.0, for the hemolymph hemolymph pernyi, saline concentration of 0 the medium can be isolated within the largest possible number of bacteria in raw citrus psyllid.The bacteria subsequently identified based on their 16S rRNA genes.They were Staphylococcus xylosus, a possible novel species in Staphylococcus sp., Bacillus sp., Enterobacter sp., Curtobacterium oceanosedimentum, Microbacterium testacyeum, Stenotrophomonas maltophilia, Curtobacterium citreum. The results showed that the physiological and biochemical identification of endophytic bacteria some common physiological and biochemical characteristics consistent with the 16S rDNA sequence identification.