SUMO2/3通过内质网途径诱导肝癌细胞凋亡
SUMO2/3 Induces Apoptosis of Hepatoma Cells through Endoplasmic Reticulum Pathway
目的 探讨小泛素相关修饰蛋白2/3（Small ubiquitin-related modifier 2/3，SUMO2/3）诱导肝癌细胞凋亡的可能机制。方法 体外培养人肝细胞癌细胞株SMMC-7721，免疫荧光染色观察SUMO2/3蛋白的表达，免疫荧光双标观察过表达或敲低SUMO2/3与活化的caspase-3的关系；利用Western Blot法检测细胞凋亡标志性蛋白caspase-3和内质网（Endoplasmic reticulum, ER）应激相关蛋白的变化。结果 SUMO2/3蛋白在肝癌细胞的细胞核中稳定表达，ER 应激诱导剂衣霉素(Tunicamycin, TM)刺激后SUMO2/3表达量增加。过表达SUMO2/3可诱导肝癌细胞凋亡，通过检测ER 应激相关蛋白的水平，发现肝癌细胞过表达SUMO2/3上调了Bip、p-PERK、p-EIF2α、ATF6、ATF4、CHOP蛋白水平。结论 SUMO2/3可能是通过ER途径诱导肝癌细胞凋亡。?????
Our previous study showed that SUMO2/3 over-expression inhibited the proliferation of hepatoma cells, but the mechanism is still unknown. In this paper, we observed SUMO2/3 expression and found that endoplasmic reticulum (ER) stress inducer Tunicamycin (TM) increased SUMO2/3 protein levels in the nuclei of hepatoma cells. Meanwhile, we showed that SUMO2/3 over-expression increased cleaved caspase-3, Bip, p-PERK, p-EIF2α, ATF6, ATF4 and CHOP levels. These results suggested that SUMO2/3 induces apoptosis of hepatoma cells through ER pathway.
王鹏、沈玉君、沈玉先、刘珺、方圣云、沙曼琪、冯利杰
基础医学分子生物学肿瘤学
肝癌SUMO2/3凋亡内质网应激
hepatoma carcinomaSUMO2/3apoptosisendoplasmic reticulum stress
王鹏,沈玉君,沈玉先,刘珺,方圣云,沙曼琪,冯利杰.SUMO2/3通过内质网途径诱导肝癌细胞凋亡[EB/OL].(2015-12-16)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201512-872.点此复制
评论