小鼠乳腺中胰岛素样生长因子及其受体的表达

In this research, some time points were set in all stages of mouse mammary gland development Double staining mammary tissue slices was examined using a laser scanning confocal microscope. The results of localization and quantification of Insulin-like growth factors and their receptor(IGF-IR) revealed that: ⑴ IGF-I played a vital role on duct branching and lobuloalveolar morphogenesis during virgin and early pregnant mammary gland, and useless to initiate lactation but useful to maintain lactationg; ⑵ IGF-II played a key role on duct morphogenesis and alveolar development, especially was important to lobuloalveolar morphognesis and differentiation during pregnant mammary gland. The high level expression of IGF-II in lactating mammary gland appeared to be critical for promoting lactation and maintaining milk production.⑶ IGF-IR expressed in all stages of mammary gland development, especially highly expressed in pregnance and later lactaion, distributed in ducts, alveolar, matrix and adipocytes, which displayed that IGFs promoted ductal morphogenesis and alveolar development, maintained lactation by IGF-IR.