稳定表达乙肝病毒X蛋白肝癌细胞系的建立及其侵袭性研究

Objective To establish a sustaining HBx protein expressed cell lines and to study the characterization of their invasive potential. Methods With the eukaryotic expression vector pcDNA3.1 and the constructed pcDNA3.1-HBx plasmid, gene transfection mediated by lipofectamine was used to introduce the eukaryotic expression vector of HBx gene into human liver cell line CCL13, and detect the stabilizing expression of HBx gene. To study the characterization of their invasive potential by experiment in vivo and in vitro. Results HBx gene was cloned from the transfected CCL13 cell line by polymerase chain reaction technique, and the stabilizing human liver cell lines CCL13 containing HBx gene expression have the characterization of malignant cell was verificated than the contrast by means of detecting their growth curve, colony forming efficiency, wound healing and transwell migration assays. Conclusion The stabilizing human liver cell lines CCL13 containing HBx gene expression have been established successfully, and its invasion ability has conditionality.