Age and diet modulate the insulin-sensitizing effects of exercise: a tracer-based oral glucose tolerance test
Age and diet modulate the insulin-sensitizing effects of exercise: a tracer-based oral glucose tolerance test
Abstract Diet modulates the development of insulin resistance during aging. This includes tissue-specific alterations in insulin signaling and mitochondrial function, which ultimately affect glucose homeostasis. Exercise stimulates glucose clearance, mitochondrial lipid oxidation and enhances insulin sensitivity. It is not well known how exercise interacts with age and diet in the development of insulin resistance. To investigate this, oral glucose tolerance tests (OGTT) with a tracer were conducted in mice ranging from 4 to 21 months of age, fed a low- (LFD) or high-fat diet (HFD), with or without life-long voluntary access to a running wheel (RW). We developed a computational model to derive glucose fluxes, which were commensurate with independent values from steady-state tracer infusions. Both insulin sensitivity indices derived for peripheral tissues and liver (IS-P and IS-L, respectively) were steeply decreased by aging and a HFD. This preceded the age-dependent decline in the mitochondrial capacity to oxidize lipids. In LFD young animals, RW access enhanced the IS-P concomitantly with the muscle β-oxidation capacity. Surprisingly, RW access completely prevented the age-dependent IS-L decrease, but only in LFD animals. This study indicates, therefore, that endurance exercise can improve the age-dependent decline in organ-specific IS mostly in the context of a healthy diet.
van Dijk Gertjan、Koshian Serj、Ciapaite Jolita、Bandsma Robert H.J.、Groen Albert K.、Bakker Barbara M.、Talarovicova Alzbeta、Wolters Justina C.、Vieira-Lara Marcel A.、Reijngoud Dirk-Jan、van Dijk Theo H.、Reijne Aaffien C.、Versloot Christian J.、Abegaz Fentaw
Groningen Institute for Evolutionary Life Sciences, Department of Behavioral Neuroscience, University of GroningenLaboratory of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of GroningenLaboratory of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of GroningenLaboratory of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of Groningen||Translational Medicine, Peter Gilgan Centre for Research and Learning, The Hospital for Sick ChildrenDepartment of Vascular Medicine, Amsterdam University Medical CentersLaboratory of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of GroningenLaboratory of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of Groningen||Groningen Institute for Evolutionary Life Sciences, Department of Behavioral Neuroscience, University of GroningenLaboratory of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of GroningenLaboratory of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of GroningenLaboratory of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of GroningenDepartment of Laboratory Medicine, University of Groningen, University Medical Center GroningenLaboratory of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of Groningen||Groningen Institute for Evolutionary Life Sciences, Department of Behavioral Neuroscience, University of GroningenLaboratory of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of Groningen||Translational Medicine, Peter Gilgan Centre for Research and Learning, The Hospital for Sick ChildrenLaboratory of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of Groningen
基础医学生物科学理论、生物科学方法生物科学研究方法、生物科学研究技术
oral glucose tolerance testcompartment modelinsulin sensitivitytracerphysiology
van Dijk Gertjan,Koshian Serj,Ciapaite Jolita,Bandsma Robert H.J.,Groen Albert K.,Bakker Barbara M.,Talarovicova Alzbeta,Wolters Justina C.,Vieira-Lara Marcel A.,Reijngoud Dirk-Jan,van Dijk Theo H.,Reijne Aaffien C.,Versloot Christian J.,Abegaz Fentaw.Age and diet modulate the insulin-sensitizing effects of exercise: a tracer-based oral glucose tolerance test[EB/OL].(2025-03-28)[2025-05-25].https://www.biorxiv.org/content/10.1101/2023.03.18.533083.点此复制
评论