N型乙酰胆碱受体α10亚基在大鼠内耳耳蜗、前庭中的表达

objective: To investigate the expression of the α10 acetylcholine receptor subunit mRNA in the rat inner ear vestibular end-organ, Scarpa’s ganglion ,cochlea basement membrane and spiral ganglion. Method: Using a combination of reverse transcription follow by polymerase chain reaction, expression of the α10 acetylcholine receptor subunit mRNA in the rat inner ear vestibular end-organ, Scarpa’s ganglion ,cochlea basement membrane and spiral ganglion was examined. The brain RNA was used as positive control. Result: PCR amplification products representing subunit gene expression of the α10 acetylcholine receptor subunit was amplified in vestibular end-organ and cochlea basement membrane. Conclusion: The result showed thatα10 acetylcholine receptor subunit mRNA is expressed in the rat vestibular end-organ and cochlea basement membrane, but in the Scarpa’s ganglion and spiral ganglion, indicating that acetylcholine is one of the important neurotransmitters in the vestibular system, and provide a more complete understanding of the role of nAChR in the neurochemical transmission subserving the efferent-afferent interacation in the inner ear.