海岛棉GbRvd基因的克隆与黄萎病菌胁迫下的表达分析

Based on a full-length cDNA library of Pima90-53(Gossypium barbadense) under Verticillium dahliae stress, a novel CC-NBS-LRR gene was amplified by in silico cloning and RACE technology, it was named GbRvd. The bioinformatics analysis showed that the full cDNA was 3 486 bp in length with an open reading frame (ORF) of 2 928 bp encoding a protein of 975 amino acids. The theoretical molecular weight and isoelectric point of GbRvd protein was 112.6 kD and 6.51，respectively. GbRvd, contains domains of NB-ARC, Smc, PLNOO113 and PLN3210, are assumed to be involved in the recognition for Verticillium dahliae effector. Quantitative real time PCR analysis indicated that the expression level of GbRvd was higher in pima90-53 than in other Gossypium hirsutum cultivars after inoculation with V. dahlia, but no significant difference in G. hirsutum. GbRvd expressed in all tissues of root, stem and leaf, whereas the highest expression was found in leaves of pima90-53 under V. dahliae stress.