融合疫苗在NOD/XCID人免疫重建鼠中抗肾癌的作用

Objective: through human cord blood stem cell transplantation in NOD / SCID mice to reconstituted human immune system, and to study the mechanism of DC fusion vaccine anti-tumor in immunized reconstruction mice , and to explore the DC fusion vaccine anti-tumor effects . Methods: The NOD / SCID mice were divided into ABCDEF6 group, which BCDEF5 group rebuild human immune system then CDEF4 group Construction A498 (kidney cancer) tumor-bearing model in skin. CDE three groups were divided given the CD, GM-CSF modified fused cells, GM-CSF-B7.1 modified fused cells for treat tumor models, followed by monitoring changes in immune function between the groups and to the size of the tumor models. Results: The Mice in immunized construction group were detect human immune cells: CD3, CD45, CD56, and the numbers of human immune cell increase with time prolonged, peaked in the eighth week, then gradually decline. The number of immune cells in immune reconstitution group were descendants than immune reconstitution group .but after applying a DC fusion vaccine therapy, the number of human immune cells increased, and DC fusion vaccine significantly reduced the size of the kidney tumor tissue. Conclusion: Determine the DC fusion vaccine with enhanced immunity, and has a strong anti-tumor effect. Prove specific modifications of fusion vaccine has significantly improved of anti-tumor effect.