罗汉果多糖对环磷酰胺所致的免疫抑制小鼠免疫功能的影响

In order to study the activity of Siraitiagrosvenorii polysaccharides(SGP), specially its immunoregulatory activity. Kun-ming mice were randomized into six groups, including normal group, model group, SGP groups(25 mgkg-1, 50 mgkg-1, 100 mgkg-1)and levamisole group. Mice were injected with cyclophosphamide (20 mgkg-1)to establish the immunosuppressive model, then all groups of mice were treated for sequential 14 days by giving different dose of SGP. In this research, effects of SGP on immune function of immunocompromised mice was studied by detecting many indices, such as thymus and spleen indices, carbon clearance index, phagocytic index. some indices liked ear edema, the concentration causing 50% hemolysis (HC50), the proliferation ability of T cells and B cells, the contents of IgG, IgM, IL-2, IL-4, IL-6 , TNF- were also be very impotant parameters to measure immune function. Histopathological examination of spleen tissue also been observing. Experiments found that SGP groups(25 mgkg-1, 50 mgkg-1, 100 mgkg-1)was significantly in improving the thymus and spleen indices. The concentration causing 50% hemolysis (HC50) was increased effectively. The level activity of serum hemolysin was rising compared to model group. Datas also showed that SGP groups(25 mgkg-1, 50 mgkg-1, 100 mgkg-1)could significantly reduce ear edema. Experiments showed that the proliferation ability of B cells, the contents of IgG, IgM, IL-2, IL-4, IL-6,TNF- were improving. SGP groups also50 mgkg-1, 100 mgkg-1could significantly increase the carbon clearance index and phagocytic index, enhance the proliferation ability of T cells. Spleen tissue damage induced by cyclophosphamide was ameliorated according to the histopathological examination. This research comed to the conclusions that SGP could significantly improve immune function of immunocompromised mice.