Rapid base-specific calling of SARS-CoV-2 variants of concern using combined RT-PCR melting curve screening and SIRPH technology
Rapid base-specific calling of SARS-CoV-2 variants of concern using combined RT-PCR melting curve screening and SIRPH technology
ABSTRACT The emergence of novel variants of concern of SARS-CoV-2 demands a fast and reliable detection of such variants in local populations. Here we present a cost-efficient and fast workflow combining a pre-screening of SARS-CoV-2 positive samples using RT-PCR melting curve analysis with multiplexed IP-RP-HPLC-based single nucleotide primer extensions (SIRPH). The entire workflow from positive SARS-CoV-2 testing to base-specific identification of variants requires about 24 h. We applied the sensitive method to monitor the local VOC outbreaks in a few hundred positive samples collected in a confined region of Germany.
J?rn Walter、Markus Vogelgesang、Stefan Lohse、Sigrun Smola、Christina Lo Porto、Beate Schmitt、Thorsten Pfuhl、Sascha Tierling、Kathrin Kattler、Abdulrahman Salhab
Department of Genetics, Saarland UniversityInstitute of Virology, Saarland University Medical CenterInstitute of Virology, Saarland University Medical CenterInstitute of Virology, Saarland University Medical CenterDepartment of Genetics, Saarland UniversityDepartment of Genetics, Saarland UniversityInstitute of Virology, Saarland University Medical CenterDepartment of Genetics, Saarland UniversityDepartment of Genetics, Saarland UniversityDepartment of Genetics, Saarland University
医学研究方法基础医学生物科学研究方法、生物科学研究技术
SARS-CoV-2Variants of concernSNuPESIRPHmutation screening
J?rn Walter,Markus Vogelgesang,Stefan Lohse,Sigrun Smola,Christina Lo Porto,Beate Schmitt,Thorsten Pfuhl,Sascha Tierling,Kathrin Kattler,Abdulrahman Salhab.Rapid base-specific calling of SARS-CoV-2 variants of concern using combined RT-PCR melting curve screening and SIRPH technology[EB/OL].(2025-03-28)[2025-06-12].https://www.medrxiv.org/content/10.1101/2021.03.15.21253586.点此复制
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