季节变化对青海沙蜥成体神经发生影响的研究

Objective: The aim of present study is to investigate the seasonal influence on the adult neurogenesis in Phrynocephalus przewalskii -- typical reptile in northwest of China, and analyze the corresponding mechanism. Among the total, adult neurogenesis in the Phrynocephalus przewalskii during hibernation was the key point we focus on. Methods: Lizards were captured during Match, July and September, respectively. According to surviving time the animals were divided into three groups: survivied 24 hours group, 7 days group, and 28 days group. The lizards that captured in September were used for the study of hibernation. Intraperitonial BrdU-injection, Immunocytochemistry and laser scanning confocal microscope were used to study the adult neurogenesis in the Phrynocephalus przewalskii during different seasons. Results: The adult neurogenesis is regulated in a seasonal way in the Phrynocephalus przewalskii obviously. While Autumn has the strongest adult neurogenesis, followed by Summer, Spring, and Winter. During the hibernation, adult neurogenesis still existence in the Phrynocephalus przewalskii, but was weak.In each season, variations was showed in different living time.