海南粗榧内生真菌Alternaria sp.次级代谢产物的分离与鉴定
Isolation and identification of secondary metabolites by endophytic fungi Alternaria sp. from Cephalotaxus hainanensis Li
目的]研究海南粗榧内生真菌Alternaria sp. CH1307c次级代谢产物。[方法]对该菌以大米固体发酵方式进行培养，采用硅胶柱层析、制备薄层层析、重结晶以及半制备液相等方法，从培养物的乙酸乙酯萃取相中分离、纯化获得8个单体化合物，运用现代波谱技术对其结构进行解析，鉴定这些化合物分别为Pestalotiopin A(1)、β-Sitosterol(2)、Alternariol 5-O-sulfate(3)、Alternariol monomethyl ether(4)、scopoletin(5)、cerevisterol(6)、oblongolide V(7)和3-Methoxyphenoxy(8)。所有化合物均为首次从海南粗榧内生真菌中分离得到。
[Objective] Study on secondary metabolites of endophytic fungi Alternaria sp. CH1307c from Cephalotaxus hainanensis Li. [Method] 8 compounds were isolated from the ethyl acetate extract of the rice fermentation by using silica gel column chromatography, preparative thin-layer chromatography, recrystallization and semi preparative liquid chromatography method, The structures of these compounds were identified by comprehensive spectroscopic characterizations as Pestalotiopin A(1), β-Sitosterol(2), Alternariol 5-O-sulfate(3), Alternariol monomethyl ether(4), Scopoletin(5), Cerevisterol(6), Oblongolide V(7) and 3-Methoxyphenoxy(8). All compounds were isolated from endophytic fungi of Cephalotaxus hainanensis Li for the first time.
徐传标、周偏、李从发、刘四新、林淑婷、刘翊昊、李培、胡晓苹
生物科学研究方法、生物科学研究技术生物化学微生物学
海南粗榧内生真菌次级代谢产物分离纯化化合物结构鉴定
ephalotaxus hainanensis LiEndophytic fungiSecondary metabolitesIsolation and purificationCompound structure identification
徐传标,周偏,李从发,刘四新,林淑婷,刘翊昊,李培,胡晓苹.海南粗榧内生真菌Alternaria sp.次级代谢产物的分离与鉴定[EB/OL].(2016-05-10)[2025-08-06].http://www.paper.edu.cn/releasepaper/content/201605-134.点此复制
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