琯溪蜜柚汁胞粒化过程中基因差别表达cDNA的克隆及序列分析

Guanxi-miyou fruit being taken as experimental material in this study, CDNA difference fragment of non-granulated juicy sac was isolated in the process of granulation of Guangxi-miyou by reverse transcription-polymerase chain reaction (RT- PCR). It was found that the Guanxi-miyou CDNA fragment was significantly homologous to developing pear fruit’s by Blast analysis. The 5’ end sequence was also found by 5’ RACE , and then the full length of CDNA given by sequence analysis, Blast and joining was analyzed, and the primary exploration on granulation was made in prospective of biology. The result showed that the full length of cDNA in Guangxi-miyou juicy sac was an open reading frame of 1152 bp including one ORF with 819 bp, and 272 amino acid sequence. The further Blast analysis showed that CDNA had highly homologous to 20S proteasome beta subunit, among which 83.09% of homology to spinach’ was highest, 74.73% to rice’ and 73.12% to wheat’ respectively.