一株极端嗜盐曲霉的分离鉴定及生物学特性分析

We isolated and identified an extreme halophilic strain CCHA from the surface of wild vegetation around soalar saltern, and identified by the morphological characters and 5.8S rDNA sequence analysis, at the same time, their biological characteristics, salt-resistance and metal-tolerance was analyzed. 5.8S rDNA sequence analysis showed that the homology of CCHA and 21 Aspergillus strains reported was 55.9%-100 %. This indicates that CCHA belongs to Aspergillus sp.. Strain CCHA grew at temperature from 25 ℃ to 45 ℃; Growth pH range was from 2.5 to 12.0; Temperature 35 ℃, pH from 5.0 to 8.0, and salt concentration 5% was the optimum culture condition. At room temperature, the strain could survive on PDA up to 500.0 g / L NaCl; At 35 ℃, It could survive two months in PDA containing 310.0 g / L NaCl. It had higher tolerance to Cu2+. In addition, it has no potential pathogenicity to rice、soybean、tobacco and cucumber. CCHA strain was identified as Aspergillus sp. with extreme halophilic characteristics and adapt to wide range of temperature and pH, being important potential application value.