P38介导Wnt6促人牙乳头细胞分化的作用机制

Objective: to study the effect of p38 on the differentiation promoted by Wnt6 in HDPCs. Methods: PGK-Wnt6 overexpression lentivirus was constructed; infected HDPCs with Wnt6 lentivirus overexpressed Wnt6; the proliferation and apoptosis of HDPCs influenced by Wnt6 virus were not changed obviously; the phosphorylation of p38 activated by Wnt6 was determined by Western blot; Alizarin red, ALP staining and qPCR were used to verify whether SB203580, a p38 inhibitor, can block the differentiation of HDPCs promoted by Wnt6. Results: The Wnt6 overexpression model in HDPCs was established; there were no obvious changes of HDPCs proliferation after infected with PGK-Wnt6 lentivirus; SB203580 treatment significantly reduced increased mineralization and the expression of osteogenic genes, including ALP and Col I, which were induced by Wnt6, but OC and DSPP have no significant changes. Conclusion：p38 mediates the early differentiation of HDPCs promoted by Wnt6.