不同培养基对人牙周韧带细胞生物学行为的影响
Effects of α-MEM,DMEM-LG and RPMI1640 culture medium on the biological behavior of human periodontal ligament cells
目的：研究α-MEM、DMEM-LG、RPMI1640三种培养基对人牙周韧带细胞（human periodontal ligament cells, hPDLCs）生物学行为的影响，为体外培养人牙周韧带细胞寻找合适的培养基。方法：采用改良组织块培养法，分别用α-MEM、DMEM-LG、RPMI1640三种培养基对hPDLCs进行体外培养，比较三组细胞在游出成功率、增殖及矿化等方面的差异。结果：α-MEM促进hPDLCs增值作用最强。α-MEM组培养的细胞表达的碱性磷酸酶（Alkaline phosphatase，ALP）含量最高，与其它两组间有显著差异。DMEM-LG组矿化结节形成个数最多且体积大。结论：α-MEM促进hPDLCs增殖，DMEM-LG促进hPDLCs分化。应根据实验要求选择合适培养基。
Objective: To observe the effects of three different culture medium: α-MEM、DMEM-LG and RPMI1640 on the biological behavior of human periodontal ligament cells(hPDLCs), and provide the best culture medium for cultured hPDLCs in vitro. Methods: hPDLCs were cultured in α-MEM、DMEM-LG and RPMI1640 respectively in vitro with the improved tissue block culture method. The successful cell migration rate, proliferation, mineralized nodules of three groups cells were defected and compared. Results: α-MEM significantly increased hPDLCs proliferation. In addition,α-MEM strongly induced alkaline phosphatase activity. But DMEM-LG group exhibited the most mineralized nodules with biggest size. Conclusion: α-MEM increased hPDLCs proliferation. DMEM-LG promoted the differentiation of hPDLCs. It could be ascertained to choose appropriate culture medium according to the demand of different experiments.
闫福华、李艳芬、唐焜琪、钟泉、姚丽艳、李大兰
基础医学细胞生物学口腔科学
牙周病学人牙周韧带细胞培养基生物学活性
PeriodontologyHuman periodontal ligament cellsCulture mediumBioactivities
闫福华,李艳芬,唐焜琪,钟泉,姚丽艳,李大兰.不同培养基对人牙周韧带细胞生物学行为的影响[EB/OL].(2011-05-05)[2025-08-04].http://www.paper.edu.cn/releasepaper/content/201105-89.点此复制
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