电针对大鼠脊髓损伤后notch1、ps1表达的实验研究

Objective：To discuss the protective mechanism of electroacupuncture (EA) in the spinal cord injury(SCI). Methods： Rats were divided into four groups randomly：Model Group，Blank Group，Drug Group and EA Group. The SCI rat model was made by using a modified Allen’s method. We will take the spinal cord tissues on the six hour, the first day and the seven day after injury. The injured tissues of Model Group，Blank Group，Drug Group and EA Group were removed to determine the content of Notch1、ps1. Total RNA was extracted，through RT-PCR.. Results： After the SCI rat model be made suceessfully，the Notch1、ps1 mRNA content of injury local tissues were decreased significantly in EA Group and Drug Group on the 6h, 1 day and the 7 day after SCI. Compared with the model Group，statistical significance were found in the EA Group and Drug Group（P<0.05）. The Notch1、ps1 mRNA content of injury local tissues were higher slightly in EA Group than the Drug Group and there was no statistical significance between the Drug Group and the EA Group（P>0.05）. Conclusion：Notch signaling pathway plays an important role in cell growth, development, differentiation and apoptosis. The activation of Notch signaling can reversibly accelerate the NSC changing into glial cell.The electric acupuncture on spinal cord injury can inhibit the expression of Notch1, ps1 significantly.