中心体Centrin蛋白在单环刺螠精子分化及早期发育过程中的表达研究

In this study, expression of centrin during Urechis unicinctus spermatid defferentiation is analyzed with anticentrin polyclone antibody. The results show not only that expression of centrin is present through whole differential process from early and late spermatid to sperm., but also that during spermatid defferentiation, centrin labeling spots changed from two spots in sperm cell to one spot in sperm.Futhermore, using indirect immunofluorescence to determine the centrin localization in trochophore cell and we found that many cluster centrin spots locate at cytoplasm. In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus; (2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora. we think that centrin play an important role in meiosis during Urechis unicinctus sperm metamorphosis difference and we also think that centrin may be related to trochophore cilia and sperm tail formation,and reconstraction of centrosome after fertilization.